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Group 1: Normal Control (NC) Group 2: High Cholesterol Diet Control (HCD), animals receiving an atherogenic food for 50 days Group 3: Animals receiving high cholesterol diet for 50 days, after this period the animals are fed for eight days by the standard food and receiving by gavage virgin olive oil (1% of body weight) (HCD+VOO)

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Fluorescence imaging of EPS secretion Actively motile O

Disclosure The authors report no conflicts of interest in this work

GHK-Cu cycling - typically 30 days on / 14 off or 8 weeks on / 4 off - produces comparable results, reduces cost, limits theoretical copper load and keeps receptor sensitivity fresh